On September 1st 1462 the fleet of Mahmud Pasha besieges Mytilene. Melanoudi falls (an area protected by outer walls on the outskirts of the city) and Niccolo Gattilusio handed over the castle. Molyvos is the next city to fall, followed by Aghii Theodori and Eresus. Ten thousand are sent to Constantinople. The island now belongs to the “Eyalet of the Island and of the Caspian Sea”. The Ottoman Turks extend and renovate the Castles of Molyvos and Mytilene. They build the Castle of Sigri (1757). The other fortresses are abandoned. The village populations have gone in hiding in gullies for fear that there may be piracy. The Monasteries are abandoned. They become state property. They are gradually bought back by Christians to be re-established (Limonos, Ipsilou, Myrsiniotissas, Pithari, Kriokopou etc.). The churches are small, dark and decorated with icons. Water mills, drinking fountains, olive mills and, during the middle of the 19th century, steam-driven olive presses are the basis of the island economy. There are two diocese one of which is transferred to Kalloni. Mosques and minarets are erected.

The beginning of the 16th century (Limonian School) marks the first cautious steps in the field of education. Towers are built all over the island. After the Russo-Turkish War (1768-1774) the Ku çu k Kaynarca Treaty was signed, establishing the protection of the Christian religion. The Hatti Humayun edict (1839-1856) establishes the principles of equality before the law, freedom of religion. It is during this period that the magnificent churches and Manor houses we see today are built. Trade is taken over by Christians (Smyrna (Izmir), Odessa, Constantinople, Alexandria). Of the 140.000 inhabitants only 40.000 are Ottoman turks. Schools and large public buildings are erected.

In 1821 Papanicolis burns down a Turkish battleship in Eressos. The present-day port of Mytilene is developed. Soap and olive oil is exported across the Mediterranean. The year 1912 is approaching.